DNA sensor

Details DNA sensor DNA sensor

2005-08-04

2010-12-14

Crow, Robert T (Department: 1634)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S006120, C257S243000, C257S253000, C536S024300

Reexamination Certificate

active

07851205

ABSTRACT:
A DNA sensor including a p-channel field-effect transistor having as a gate an electrolyte solution and having as a channel a diamond surface which contains a mixture of at least a hydrogen-terminated surface and a surface terminated by an amino group or a molecule with an amino group as an amino termination; a probe DNA constituted of a single-stranded DNA with known nucleotide sequence which is directly immobilized by a linker to the amino termination of the diamond surface; and a target DNA constituted of an unknown single-stranded DNA which is dropped on said diamond surface, wherein the hybridization of the target and probe is ascertained by detecting a shift of the threshold voltage of said p-channel field effect transistor toward positive direction which is due to increase in hole density of the p-channel resulting from doubling the negative electric charge of the phosphate groups upon hybridization.

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Hideo Hata, “DNA Attachment and Hybridization At the Diamond Surface”, Dai 50 Kai Oyo Butsurigaku Kankei Rengo Koenkai Koen Yokoshu, vol. 2, p. 167, 2003.
Hiroshi Kawaharada, “Kinosei Diamond Hyomen Eno Seitai Bunshi Kotei Oyobi Biosensor Oyo” Waseda Daigaku Coe ‘Bunshi Nano Kogaku’ Kenkyu Kyoten, ‘Nano Kozo Hairetsu O Kiban Tosuru Bunshi Nano Kogaku No Kochiku to Microfilm System Eno Tenkai’ Jikken Hokokusho, pp. 161-162, 2004.

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