DNA segments and methods for increasing polysaccharide productio

Details DNA segments and methods for increasing polysaccharide productio DNA segments and methods for increasing polysaccharide productio

1996-01-24

1998-12-29

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 72, 435104, 4352523, 536 232, C12P 1904, C12P 1906, C12P 121, C12P 2104, C12N 121, C07N 2104

Patent

active

058540342

ABSTRACT:
The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.

REFERENCES:
patent: 4326053 (1982-04-01), Kang et al.
patent: 5338841 (1994-08-01), Pollock et al.
Martins, L.O. et al. "Gellan gum biosynthetic enzymes in producing and nonproducing variants of Pseudomonas elodea." Biotechnology and Applied Biochemistry, vol. 14, No. 3 (1991), pp. 1357-364 1991.
Harding, N.E. et al. "Isolation of genes essential for the biosynthesis of gellan gum." FASEB Journal, vol. 7, No. 7 (Apr., 1993), p. A1259.
Pollock, T.J. "Gellan-related polysaccharides and the genus Sphingomonas" Journal of General Microbiology, vol. 139, (1993), pp. 1939-1945.
Alberts, B. et al. Molecular Biology of the Cell, Second Edition. N.Y., Garland Publishing, 1989. pp. 258-266.
Martin, M.O. "Synthesis of commercially valuable bacterial polymers: impact of molecular genetics." Research in Microbiology, vol. 145, (1994), pp. 93-97.
Fialho, A.M. et al. "Conjugal transfer of recombinant plasmids into gellan gum-producing and non-producing variants of Pseudomonas elodea ATCC 31641." Letters in Applied Microbiology, vol. 12, (Mar. 1991), pp. 85-87.

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