DNA segment containing streptomycin resistance gene and being ca

Details DNA segment containing streptomycin resistance gene and being ca DNA segment containing streptomycin resistance gene and being ca

1991-02-25

1993-04-13

Martinell, James

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4351723, 4353201, 43525235, 935 14, 536 232, C12N 1552, C12N 1531, C12N 1500, C12N 1574

Patent

active

052024270

ABSTRACT:
A DNA segment of this invention is a DNA fragment of a length of about 3.8 kb which is obtained by excising with a restriction endonuclease Bgl II a hybrid plasmid pST 141 having a length of about 12.6 kb of Streptomyces griseus 4-1 strain (FERM P-7984, namely, FERM BP-1198) to give a Bgl II-Bgl II DNA fragment, and then excising with a restriction endonuclease Sph I the resultant Bgl II-Bgl II DNA fragment having a length of about 7.0 kb and having the restriction endonuclease sites as shown in the restriction endonuclease map of FIG. 1 . This DNA fragment is a DNA which contains a streptomycin resistance gene and contains, in the vicinity of this gene, such a DNA region possessing a function to control the expression of the streptomycin resistance gene. An insertion of this DNA fragment having a length of about 3.8 kb into a suitable actinomycetes plasmid vector can produce such a hybrid plasmid which acts reliably as a selected marker of the streptomycin resistance.

REFERENCES:
Distler et al.; FEMS Microbiol. Lett. 28: 113 (1985).
Tohyama et al.; Chem. Abstr. 102: 73512c (1985).
Vallins et al.; J. gen. Microbiol. 131: 1657 (1985).

Affiliated with

Also associated with

Rating

DNA segment containing streptomycin resistance gene and being ca does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA segment containing streptomycin resistance gene and being ca, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA segment containing streptomycin resistance gene and being ca will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1156311