DNA/RNA target and signal amplification

Details DNA/RNA target and signal amplification DNA/RNA target and signal amplification

1995-05-15

1996-11-12

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

055739148

ABSTRACT:
A process for detecting the presence or absence of a specific nucleic acid sequence or antibody in a sample using an oligonucleotide to bind to the nucleic acid sequence or antibody to be detected, forming double-stranded nucleic acid sequence using the bound oligonucleotide in conjunction with another oligonucleotide or DNA synthesis, synthesizing RNA transcripts from the thus-formed double-stranded nucleic acid sequence, and detecting the existence of the RNA transcripts, and oligonucleotides and kits useful in carrying out such a process.

REFERENCES:
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4749647 (1988-06-01), Thomas et al.
patent: 4786600 (1988-11-01), Kramer et al.
patent: 4851331 (1989-07-01), Vary et al.
patent: 4882269 (1989-11-01), Schneider et al.
patent: 4957858 (1990-09-01), Chu et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4994373 (1991-02-01), Stavrianopoulos et al.
patent: 5112734 (1992-05-01), Kramer et al.
patent: 5118605 (1992-06-01), Urdea
Chu, B. C. F., et al., "Synthesis of an Amplifiable Reporter RNA for Bioassays", Nucleic Acids Research, vol. 14, No. 14, pp. 5591-5603 (1986).
Diamandis, E. P., "Detection Techniques for Immunoassay and DNA Probing Applications", Clinical Biochemistry, vol. 23, pp. 437-443, especially pp. 441-442 (Oct., 1990).
Kwoh, D. Y., et al., "Target Amplification Systems in Nucleic Acid-Based Diagnostic Approaches", American Biotechnology Laboratory, vol. 8, No. 13, pp. 14-25, especially pp. 20, 24, 25 (1990).
Thompson, J. D., et al., "Current Concepts in Quantitative Hybridization", Clinical Biochemistry, vol. 23, pp. 261-266, especially p. 265 (Aug. 1990).
Guatelli et al., "Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication," Proc. Natl. Acad. Sci. USA 87:1874-1878 (1990).
Kwoh et al., "Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format," Proc. Natl. Acad Sci. USA 86:1173-1177 (1989).
Fahy et al., Self-sustained sequence replication (3SR): an isothermal transcription-based amplification system alternative to PCR, PCR Methods Appl. 1:25-33 (1991).
Resto et al., Amplification of protein expression in a cell free system, Nucl. Acids Res. 20:5979-5983 (1992).
Kramer et al. Am. Biol. Clin 48/6:409-411 (1990) "Amplifiable hybridization probes".
D. Y. Kwoh et al. Proc. Natl. Acad. Sci. USA 86, 1173-1177 (1989).

Affiliated with

Also associated with

Rating

DNA/RNA target and signal amplification does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA/RNA target and signal amplification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA/RNA target and signal amplification will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-561687