Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-04-29
2000-01-25
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104, C15N 1500
Patent
active
060177098
ABSTRACT:
Guanine-rich oligonucleotide primers and short telomeric DNA oligonucleotides that serve as non-conventional templates for the synthesis of long, nascent DNA strands are used to screen for pharmaceutical agents. Using the methods of the invention, agents exhibiting antagonistic or agonistic effects on guanine quartet formation can be selected for further study. The invention also provides a predictive assay for cancer in which the level of DNA polymerase activity at a quartet stabilized template is measured. The invention also contributes a method for screening pharmaceutical agents that reduce or inhibit DNA polymerase activity at guanine quartet stabilized templates. The invention provides a method of creating extended DNA molecules from oligonucleotide templates. The invention also provides a method of inhibiting the ageing process in which an oligonucleotide capable of forming non-Watson-Crick structures is used to promote the elongation of telomeres by DNA polymerase.
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Hardin Susan Houck
Jones Leslie Burgan
Ying Jun
Marschel Ardin H.
University of Houston
Whisenant Ethan
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