DNA purification procedure

Details DNA purification procedure DNA purification procedure

1996-07-23

1998-07-07

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 411, C12P 1934, C07H 2100

Patent

active

057770980

ABSTRACT:
A process and apparatus for purifying DNA which has relatively high molecular weight from a biological sample containing DNA is described. The method comprises the steps (1) lysing the red blood cells in the sample; 2) lysing DNA-containing cells; (3) precipitating the proteins from sample; and (4) and rehydrating and recovering the DNA. method offers the advantages of performance in less than 15 minutes, not requiring organic or poisonous materials, compatibility with most anticoagulants, and requiring less solutions than conventional purification procedures, thus making it simpler and more economical to use.

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Miller et al. Nucl. Acid Res. 16(3):1215-1217, 1988.

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