Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Patent
1982-09-27
1985-05-21
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
435 68, 435 70, 4351723, 435253, 435317, 536 27, 935 8, 935 32, 935 73, C12P 2104, C12P 2100, C12P 2102, C12N 1500, C12N 120, C12N 100, C07H 1512
Patent
active
045186901
ABSTRACT:
A molecule of double-stranded DNA having a length of about 35 base pairs has been removed from the first 150 base pairs at the 3'-end of Avian sarcoma viral DNA. This molecule binds to E. coli RNA polymerase and acts as a promoter of gene expression in E. coli. E. coli cells have been transformed with cloning vehicles which include this promoter DNA molecule and enhanced gene expression has been observed in the transformed cells. By including the promoter DNA molecule in cloning vehicles along with a gene associated with the production of a desired chemical product, such as proinsulin, the polypeptide A or B chains of insulin, the polypeptide portion of interferon, a growth hormone, an enzyme or an antibody, it will be possible to obtain enhanced production of such desired chemical products.
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The invention described herein was made in the course of work under Contract Number N01 CP71055 and Grant Number 1 K04 CA 00540-01 from the Department of Health, Education and Welfare.
Research Corporation
Tanenholtz Alvin E.
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