Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1989-06-16
1991-12-03
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436 94, 436501, 536 26, 536 27, 536 28, C12Q 168, G01N 33566, C07H 1512, C07H 1700
Patent
active
050700111
ABSTRACT:
This invention provides DNA probes which are useful for the detection of Haemophilus ducreyi. This invention also provides a method of detecting in a subject the presence of H. ducreyi which comprises obtaining a suitable sample from the subject, denaturing the DNA in the sample so as to produce single stranded DNA molecules, contacting the single stranded DNA molecules so obtained with a single stranded oligonucleotide probe, under suitable conditions which permit hybridization of complementary single stranded molecules, and detecting the presence of hybridized molecules, whereby the presence of H. ducreyi in the subject is detected. This invention further comprises a kit which is useful for the detection of H. ducreyi.
REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4374925 (1983-02-01), Litman et al.
patent: 4740467 (1988-04-01), Kettman et al.
patent: 4810644 (1989-03-01), Tchen et al.
Bopp Lawrence
Parsons Linda M.
Shayegani Mehdi
Waring Alfred L.
Health Research , Inc.
Steffe Eric
Wax Robert A.
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