DNA probe which reveals a hypervariable region on human chromoso

Details DNA probe which reveals a hypervariable region on human chromoso DNA probe which reveals a hypervariable region on human chromoso

1987-05-22

1992-04-07

Wax, Robert A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 4351723, 436501, 935 78, C07H 2104, C12Q 168, G01N 3353

Patent

active

051029960

ABSTRACT:
A DNA probe p13-1-25 is homologous to at least a portion of a hypervariable DNA region located on chromosome 19p13.2.fwdarw.19cen in the human genome. The DNA region displays extensive restrictive fragment length polymorphisms when digested with certain restriction endonucleases. Probe p13-1-25 is believed to have three closely linked lock (.alpha., .phi., .epsilon.). Loci .alpha. and .phi. each have two common alleles, whereas .epsilon. has at least 33 alleles, including a null allele. Unrelated individuals display unique fragment patterns on TaqI blots probed with p13-1-25. The probe can be used to produce a genetic "fingerprint" for establishing human identity, determining engraftment of bone marrow transplants, determining parentage, and otherwise mapping genes.

REFERENCES:
patent: 4302204 (1981-11-01), Wahl et al.
patent: 4468464 (1984-08-01), Cohen et al.
patent: 4594318 (1986-06-01), Gusella et al.
patent: 4623619 (1986-11-01), Owerbach et al.
Wyman et al., "A highly polymorphic locus in human DNA", Proc Natl. Acad. Sci. U.S.A., 77:6754-6758 (1980).
Jeffreys et al., "Individual-specific `fingerprints` of human DNA", Nature, 316:76-79 (1985).
Buroker et al., "A hypervariable DNA region on human chromosome lp", Genetics, 113:PT2 (Supp. 1986).
Nakamura et al., "Characterization of a human `midisatellite` sequence", Nucleic Acids Research, 15:2537-2547 (1987).
Litt et al., "A highly polymorphic locus in human DNA revealed by cosmid-derived probes", Proc. Natl. Acad. Sci., 82:6206-6210 (1985).
Litt et al., "A Highly Polymorphic Locus in Human DNA Revealed by Probes from Cosmid 1-5 Maps to Chromosome 2q35.fwdarw.37", Am. J. Hum. Genet. 38:288-296 (1986).
Bufton et al., "Four Restriction Fragment Length Polymorphisms Revealed by Probes from a Single Cosmid Map to Chromosome 19", Am J Hum Genet 38:447-460 (1986).
Buroker et al., "A Hypervariable Region at the D19S11 Locus", Human Genetics, 76:90-95 (1987).
White, R. et al., (1986) in DNA Probes, Applications in Genetic and Inf. Disease and Cancer (Ed. L. S. Lerman, Cold Spring Harbor Labs, Cold Spring Harbor, N.Y.) pp. 43-47.

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