DNA probe signal amplification

Details DNA probe signal amplification DNA probe signal amplification

1992-05-29

1995-08-01

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, C12Q 168

Patent

active

054379776

ABSTRACT:
A method for amplifying a signal during the detection of target nucleic acid molecules utilizes a primary oligonucleotide probe that binds to a bridging nucleic acid molecule. The bridging molecule hybridizes to a first developer nucleic acid molecule. Each first developer molecule comprises: (a) a first branch having a sequence of at least two different nucleotides and at least six total nucleotides complementary to a sequence of a first branch of a second developer molecule; (b) a second branch comprising a sequence of at least two different nucleotides and at least six total nucleotides complementary to a sequence of a second branch of the second developer molecule; and (c) a detectable label. In the method, the bridging molecule binds to the primary probe and hybridizes to the first developer molecule; the bound first developer molecule hybridizes to the second developer molecule to form a developer chain; additional first and second developer molecules are added to the chain; and the labeled developer molecules in the developer chain are detected.

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Horn et al., Nucleic Acids Research 17, 6959-6967 (1989).
Teng et al., Nucleic Acids Research 16, 2671-2690.

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