Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-12-01
1998-05-26
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, C12Q 168, C07H 2102
Patent
active
057562954
ABSTRACT:
DNA primers each having a nucleotide sequence substantially complementary to a nucleotide sequence which codes for Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg; methods for amplifying a polynucleotide which codes for a peptide or a precursor protein thereof using said DNA primer; screening methods for a polynucleotide which codes for a peptide comprising an amino acid sequence of Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg or a precursor protein thereof from a DNA library, etc. using said DNA; the polynucleotide obtained by said screening method; and the peptide or its precursor protein encoded by said polynucleotide, or a salt thereof. When the DNA primer of the present invention is used, the DNA which codes for a peptide having a useful physiological activity or a precursor protein thereof can be efficiently cloned.
REFERENCES:
Erlich, "PCR Technology" Principles and Applications for DNA Amplification, pp. 99-104.
Onda et al., "One of the Endothelin Gene Family, Endothelin 3 Gene, is Expressed in the Placenta", FEBS Letters, vol. 261, No. 2, pp. 327-330, Feb. 1990.
Kosaka et al., "Biosynthesis of Human Endothelins in Transformants Expressing cDNAs for Human Prepro-Endothelins", The Journal of Biochemistry, vol. 116, No. 2, pp. 443-449, 1994.
Whitfeld et al., "The Human Pro-Opiomelanocortin Gene: Organization, Sequence, and Interspersion with Repetitive DNA", DNA, vol. 1, No. 2, pp. 133-143, 1982.
Hosoya Masaki
Onda Haruo
Campbell Eggerton A.
Takeda Chemical Industries Ltd.
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