DNA polymerases with enhanced length of primer extension

Details DNA polymerases with enhanced length of primer extension DNA polymerases with enhanced length of primer extension

1995-06-07

2008-12-16

Benzion, Gary (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200

Reexamination Certificate

active

07465539

ABSTRACT:
A formulation of thermostable or other DNA polymerases comprising (a) at least one thermostable or other DNA polymerase which lacks 3′-exonuclease activity, and (b) at least one thermostable DNA polymerase exhibiting 3′-exonuclease activity, wherein the ratio of (a) to (b) is greater than 1 to 1. Also provided is an improved method for enzymatic extension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is made and used to catalyze primer extension, are also provided.

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