Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2011-03-01
2011-03-01
Chunduru, Suryaprabha (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S006120, C435S091200, C530S350000
Reexamination Certificate
active
07897738
ABSTRACT:
The present teachings relate to DNA polymerases that have an F667Y mutation as defined with respect to Taq DNA polymerase and exhibit reduced discrimination against labeled nucleotides into polynucleotides.The teachings also include kits comprising the subject DNA polymerases and numerous methods of using the subject DNA polymerases, including uses for chain termination sequencing and PCR.The present teachings relate to DNA polymerases that have an F667Y mutation as defined with respect to Taq DNA polymerase and exhibit reduced discrimination against labeled nucleotides into polynucleotides. The teachings also include kits comprising the subject DNA polymerases and numerous methods of using the subject DNA polymerases, including uses for chain termination sequencing and PCR.
REFERENCES:
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 4500707 (1985-02-01), Caruthers et al.
patent: 4668777 (1987-05-01), Caruthers et al.
patent: 4711848 (1987-12-01), Insley et al.
patent: 4795699 (1989-01-01), Tabor et al.
patent: 4801537 (1989-01-01), Nagarajan et al.
patent: 4806472 (1989-02-01), de Louvencourt et al.
patent: 4811218 (1989-03-01), Hunkapiller et al.
patent: 4873192 (1989-10-01), Kunkel et al.
patent: 4935361 (1990-06-01), Lin et al.
patent: 4946786 (1990-08-01), Tabor et al.
patent: 4966841 (1990-10-01), Riley
patent: 4973679 (1990-11-01), Caruthers
patent: 5047519 (1991-09-01), Hobbs et al.
patent: 5063158 (1991-11-01), Schoner et al.
patent: 5071743 (1991-12-01), Slilaty
patent: 5079352 (1992-01-01), Gelfand et al.
patent: 5132418 (1992-07-01), Caruthers et al.
patent: 5151507 (1992-09-01), Hobbs, Jr. et al.
patent: 5153319 (1992-10-01), Caruthers et al.
patent: 5266490 (1993-11-01), Davis et al.
patent: 5284760 (1994-02-01), Feinstone et al.
patent: 5322785 (1994-06-01), Comb et al.
patent: 5352778 (1994-10-01), Comb et al.
patent: 5354670 (1994-10-01), Nickoloff et al.
patent: 5432082 (1995-07-01), Galeotti et al.
patent: 5436149 (1995-07-01), Barnes
patent: 5455170 (1995-10-01), Abramson et al.
patent: 5489523 (1996-02-01), Mathur
patent: 5500363 (1996-03-01), Comb et al.
patent: 5541099 (1996-07-01), Chatterjee
patent: 5541311 (1996-07-01), Dahlberg et al.
patent: 5545552 (1996-08-01), Mathur
patent: 5556747 (1996-09-01), Kumar
patent: 5556772 (1996-09-01), Sorge et al.
patent: 5583023 (1996-12-01), Cerutti et al.
patent: 5602756 (1997-02-01), Atwood
patent: 5604118 (1997-02-01), Giri et al.
patent: 5614365 (1997-03-01), Tabor et al.
patent: 5770716 (1998-06-01), Khan et al.
patent: 5800996 (1998-09-01), Lee et al.
patent: 5821356 (1998-10-01), Khan et al.
patent: 5863727 (1999-01-01), Lee et al.
patent: 5885813 (1999-03-01), Davis et al.
patent: 5939292 (1999-08-01), Gelfand et al.
patent: 5939301 (1999-08-01), Hughes et al.
patent: 5945312 (1999-08-01), Goodman et al.
patent: 5948614 (1999-09-01), Chatterjee
patent: 6107032 (2000-08-01), Kilger et al.
patent: 6225092 (2001-05-01), Kilger et al.
patent: 6605428 (2003-08-01), Kilger et al.
patent: 0655506 (1995-05-01), None
patent: 0727496 (1996-08-01), None
patent: 0823479 (2004-09-01), None
patent: WO-96/12042 (1996-04-01), None
patent: WO-97/42348 (1997-11-01), None
patent: WO-03/004632 (2003-01-01), None
patent: WO-03/048307 (2003-06-01), None
patent: WO-03/048309 (2003-06-01), None
patent: WO-03/048310 (2003-06-01), None
patent: WO-03/066804 (2003-08-01), None
patent: WO-2007/076464 (2006-12-01), None
02011305.6, “European Search Report Mailed Aug. 15, 2002”, pp. 1-4.
09009374.1, “Extended European Search Report Mailed Jun. 7, 2010”, pp. 9.
10003210.1, “Extended European Search Report Mailed Jun. 8, 2010”.
Joyce, C et al., “Function and Structure relationships in DNA Polymerases”,Annual Review of Biochemistry, vol. 63, pp. 777-822 (1994).
Kalman, L. V. et al., “Thermostable DNA Polymerases With Altered Discrimination Properties”,Genome Science and Technology, Larchmont, vol. 1, pp. 42 (1995).
Kilger, C et al., “Direct DNA sequence determination from total genomic DNA”,Nucleic Acids Research, vol. 25(10), pp. 2032-2034 (1997).
Parker, L. T. et al., “AmpliTaq DNA Polymerase, FS Dye-Terminator Sequencing: Analysis of Peak Height Patterns”,BioTechniques, vol. 21(4), PJB Publications, London., 694-699 (1996).
Prober, J et al., “A system for rapid DNA sequencing with fluorescent chain-terminanting dideoxynucleotides”,Science, vol. 238(4825), pp. 336-341 (1987).
Reeve, M et al., “A novel thermostable polymerase for DNA sequencing”,Naturevol. 376, pp. 796-797 (1995).
Suzuki, M et al., “Random mutagenesis of Thermus aquaticus DNA polymerase I: Concordance of immutable sites in vivo with the crystal structure”, vol. 93(18).Proceedings of the National Academy of Sciences(PNAS), pp. 9670-9675 (1996).
Van Den Boom, D et al., “Combined amplification and sequencing in a single reaction using two DNA polymerases with differential incorporation rates for dideoxynucleotides”,J. Biochem & Biophys Methods, vol. 35, pp. 69-79 (1987).
Voss, H. et al., “Automated Cycle Sequencing with Taquenase: Protocols for Internal Labeling, Dye Primer and Doublex Simultaneous Sequencing”,BioTechniques, vol. 23(2), pp. 312-318 (1997).
Adelman, John et al., “In Vitro Deletional Mutagenesis for Bacterial Production of the 20,000-Dalton Form of Human Pituitary Growth Hormone”, DNA vol. 2(3), 1983, pp. 183-193.
Barnes, Wayne M. , “The Fidelity of Taq Polymerase Catalyzing PCR is Improved by an N-Terminal Deletion”, vol. 112, Gene, 1992, pp. 29-35.
Bernad, Antonio et al., “A Conserved 3'-5' Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases”, Cell vol. 59, 1989, pp. 219-228.
Braithwaite, Dan K. et al., “Compilation, alignment, and phylogenetic relationships of DNA polymerases”,Nucleic Acids Researchvol. 21(4), 1993, pp. 787-802.
Brandis, John W. , “Dye structure affects Taq DNA polymerase terminator selectivity”,Nucleic Acids Researchvol. 27(8), 1999, pp. 1912-1918.
Brandis, John W. et al., “Slow Rate of Phosphodiester Bond Formation Accounts for the Strong Bias that Taq DNA Polymerase Shows against 2',3'-Dideoxynucleotide Terminators”,Biochemistryvol. 35, 1996, pp. 2189-2200.
Desai, U et al., “Single-step Purification of a Thermostable DNA Polymerase Expressed in Escherichia Coli”,BioTechniquesvol. 19(5), 1995 pp. 780-784.
Fersht, Alan , “Basic Equations of Enzyme Kinetics”,Enzyme Structure and Mechanism2nd Ed, W.H. Freedman and Company, New York, 1985 pp. 111-112.
Johnson, Kenneth , “Conformational Coupling in DNA Polymerase Fidelity”,Annu. Rev. Biochem. 62, 1993, pp. 685-713.
Patel, Smita et al., “Pre-Steady-State Kinetic Analysis of Processive DNA Replication Including Complete Characterization of an Exonuclease-Deficient Mutant”,Biochemistry30 1991 , 511-525.
Smith, Lloyd et al., “Fluorescence detection in automated DNA sequence analysis”,Naturevol. 321 1986, pp. 674-679.
Zoller, M. J. et al., “Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA”,Nucl. Acids Res. vol. 10(20), 1982 , pp. 6487-6500.
Bloom Curtis
Brandis John
Richards John H.
Applied Biosystems LLC
Bortner Scott R.
Chunduru Suryaprabha
LandOfFree
DNA polymerases having improved labeled nucleotide... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA polymerases having improved labeled nucleotide..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA polymerases having improved labeled nucleotide... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2744346