DNA polymerase with proof-reading 3'-5' exonuclease activity Bac

Details DNA polymerase with proof-reading 3'-5' exonuclease activity Bac DNA polymerase with proof-reading 3'-5' exonuclease activity Bac

1995-10-18

1998-05-05

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435194, 4352525, 435 6, C12P 1934, C12N 912, C12N 120

Patent

active

057472980

ABSTRACT:
The invention relates to a thermostable Bacillus stearothermophilus DNA polymerase which is capable of proofreading 3'-5' exonuclease activity during DNA sequencing of a DNA strand, such that the DNA polymerase functions to excise mismatched nucleotides from the 3' terminus of the DNA strand at a faster rate than the rate at which the DNA polymerase functions to remove nucleotides matched correctly with the nucleotides of the template, and which DNA polymerase does not exhibit 5'-3' exonuclease activity. The invention also relates to strains of Bacillus stearothermophilus capable of producing the thermostable DNA polymerase.

REFERENCES:
Bio-Rad, U.S. Bulletin 171, Fluorescent-Labeled DNA Sequencing Reactions ng Bst Polymerase,92-1362 1192,(date not availble).
Technical Bulletin, Promega, V. Sequence of pGEM.RTM.3z(+) Vector, Revised Feb. 1995, pp. 5-6.
Michael G. Riggs, Starla Tudor, Mathoor sivaram, Sherrol H. McDonough, Construction of Single Amino Acid Substitution Mutants of Cloned Bacillus stearothermophilus DNA Polymerase Which-Lacks 5'.fwdarw.3' Exonuclease Activity, Biochemica et Biophysica Acta 1307(1996) 178-186.
Jason M. Aliotta, John J. Pelletier, Jennifer L. Ware, Laurie S. Moran, Jack S. Benner, Huimin Kong, Thermostable Bst DNA Polymerase I Lacks a 3'.fwdarw.5'Proofreading Exonuclease Activity, Genetic Analysis Biomolecular Engineering, 12(1966) 185-195.
Sanger, et al., "DNA sequencing with chain-terminating inhibitors", Biochemistry: Proc. Natl. Acad. Aci. USA 74(197). pp. 5463-5467.
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Earley, et al., "Robotic Automation of Dideoxyribonucleotide Sequencing Reactions", Research Reports, vol. 17, No. 1 (1994) 156-165.
Mardis et al., "Automated Methods for Single-Stranded DNA Isolation and Dideoxynucleotide DNA Sequencing Reactions on a Robotic Workstation", Research Report, vol. 7, No. 8 (1989) pp. 840-850.
"What's new in this catalog?", Isotherm.COPYRGT. DNA Polymerase (rBst DNA Polymerase large fragment), Epicentre Technologies Corporation, 1994, p. 1.
Bio Rad, "Premixed Nucleotide Sequencing Kits for Bst.RTM.DNA Polymerase", US Bulletin 1649, 1993.
ATCC Catalogue, Eighteenth Edition, 1992, p. 51
Kaboeu et al., J. Bact. vol. 145, No. 1, pp. 21-26, Jan. 1981.

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