DNA polymerase with modified processivity

Details DNA polymerase with modified processivity DNA polymerase with modified processivity

1996-05-31

1999-10-26

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 530350, C12Q 168, C12Q 170, C07K 100

Patent

active

059726032

ABSTRACT:
Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

REFERENCES:
patent: 4795699 (1989-01-01), Tabor
patent: 5466591 (1995-11-01), Abramson et al.
patent: 5614365 (1997-03-01), Tabor et al.
Asakura et al., "Cloning, Nucleotide Sequence, and Expression in Escherichia coli of DNA Polymerase Gene (polA) from Thermus thermophilus HB8," J. Ferment. Bioeng. 76:265-269 (1993).
Ausubel et al., Current Protocols in Molecular Biology, Wiley Interscience, New York, 16.2.2 (1990).
Beese et al., "Structure of DNA Polymerase I Klenow Frament Bound to Duplex DNA," Science 260:352-355 (1993).
Blanco et al., "A general structure for DNA-dependent DNA polymerases," Gene 100:27-38 (1991).
Bryant et al., "Elementary Steps in the DNA Polymerase I Reaction Pathway," Biochemistry 22:3537-3551 (1983).
Chaiken et al., "Analysis of Macromolecular Interactions using Immobilized Ligands," Analytical Biochemistry 201:197-210 (1992).
Huber et al., "Escherichia coli Thioredoxin Stabilizes Complexes of Bacteriophage T7 DNA Polymerase and Primed Templates," J. Biol. Chem. 262:16224-16232 (1987).
Joyce and Grindley, "Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli," Proc. Natl. Acad. Sci. USA 80:1830-1834 (1983).
Lopez et al., "Characterization of the polA Gene for Streptococcus pneumoniae and Compositions of the DNA Polymerase I It Encodes to Homologous Enzymes from Escherichia coli Phage T7," J. Biol. Chem. 264:4255-4263 (1989).
Lu et al., "Histidine Patch Thioredoxins," J. Biol. Chem. 271:5059-5065 (1996).
Malmqvist, "Biospecific Interaction Analysis Using Biosensor Technology," Nature 361:186-187 (1994).
Myers and Gelfand, "Reverse Transcription and DNA Amplification by a Thermus thermophilus DNA Polymerase," Biochemistry 30:7661-7666 (1991).
Sarkar et al., "The "Megaprimer" Method od Site-Directed Mutagenesis," BioTechniques 8(4):404-407 (1990).
Tabor and Richardson, "Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerse by in Vitro Mutagenesis," J. Biol. Chem. 264(11):6447-6458 (1989).
Yehle and Ganesau, "Deoxyribonucleic Acid Synthesis in Bacteriophage SP01-infected Bacillus subtilis, " J. Biol. Chem. 248:7456-7463 (1973).

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