Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1996-07-11
1999-07-20
Stucker, Jeffrey
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
4241861, 4242291, 435 6, 435 691, 4353201, 4352351, 514 44, 530300, 536 232, 536 2372, 536 243, A61K 3902, C07H 2104, C07K 100, C12N 900
Patent
active
059257334
ABSTRACT:
This invention provides isolated polynucleotides encoding DNA polymerases of three members of a subfamily of gamma herpes viruses. Two were obtained from macaque monkeys affected with retroperitoneal fibromatosis, the other from human AIDS patients affected with Kaposi's sarcoma. A 454-base pair fragment encoding a region near the active site of the DNA polymerase is 69-83% identical amongst the three viruses, but only 54-68% identical with other known gamma herpes sequences and <55% identical with alpha and beta herpes sequences. Also provided are polynucleotides encoding DNA polymerase from related viruses in the RFHV/KSHV subfamily. Polynucleotides prepared according to the sequence data can be used as reagents to detect and characterize related sequences. Such reagents may be used to detect members of the RFHV/KSHV subfamily, including but not limited to RFHV, RFHV2, and KSHV. Corresponding polypeptides and peptide fragments may be obtained by expressing the polynucleotide or by chemical synthesis. They may be used for detecting specific antibody potentially present in the serum of infected subjects. They may also be used for designing or screening pharmaceutical compounds that limit viral replication by inhibiting DNA polymerase activity.
REFERENCES:
patent: 5120639 (1992-06-01), Haffey et al.
patent: 5171568 (1992-12-01), Burke et al.
patent: 5223391 (1993-06-01), Coen et al.
patent: 5244792 (1993-09-01), Burke et al.
patent: 5350671 (1994-09-01), Houghton et al.
patent: 5354653 (1994-10-01), Matsumoto et al.
Martin, III et al., "Kaposi sarcoma" Medicine (1993) 72:245-261.
Finesmith et al., "Kaposi's sarcoma" Int. J. Derm. (1994) 33:755-762.
Berel et al., "Kaposi's sarcoma among persons with AIDS: a sexually transmitted infection?" Lancet (1990) 335:123-128.
Chang et al., "Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma" Science (1994) 266:1865-1869.
Ambroziak et al., "Herpes-like sequences in HIV-infected and uninfected Kaposi's sarcoma patients" Science (1995) 268:582-583.
Lisitsyn et al., "Cloning the differences between two complex genomes" Science (1993) 259:946-951.
Moore et al., "Detection of herpesvirus-like DNA sequences in Kaposi's sarcoma in patients with and those without HIV infection" New Engl. J. Med. (1995) 332:1181-1185.
Cesarman et al., "Kaposi's sarcoma-associates herpesvirus-like DNA sequences in AIDS-related body-cavity-based lymphomas" New Engl. J. Med. (1995) 332:1186-1191.
Emery et al., "Herpesviruses" Molecular and Cell Biology of Opportunistic Infections in AIDS (1992) Myint and Cann, eds., Chapman and Hall, London, pp. 257-277.
Karlin et al., "Molecular evolution of herpesviruses: Genomic and protein sequence comparisons" J. Virol. (1994) 68:1866-1902.
Giddens, Jr. et al., "Enzootic retroperitoneal fibromatosis in macaca spp." Viral and Immunological Diseases in Nonhuman Primates (1983) pp. 249-253.
Tsai et al., "Retroperitoneal fibromatosis and acquired immunodeficiency syndrome in macaques: Clinical and immunologic studies" Lab. Animal Sci. (1986) 36:119-124.
Derbyshire et al., "The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction" EMBO J. (1991) 10:17-24.
Bernad et al., "A conserved 3'.fwdarw.5' exonuclease active site in prokaryotic and eukaryotic DNA polymerases" Cell (1989) 59:219-228.
Simon et al., "The 3' to 5' exonuclease activity located in the DNA polymerase .delta. subunit of Saccharomyces cerevisiae is required for accurate replication" EMBO J. (1991) 10:2165-2171.
Soengas et al., "Site-directed mutagenesis at the Exo III motif of .phi.29 DNA polymerase; overlapping structural domains for the 3'-5' exonuclease and strand-displacement activities" EMBO J. (1992) 11:4227-4237.
Dorsky et al., "Expression of herpes simplex virus type 1 DNA polymerase gene by in vitro translation and effects of gene deletions on activity" J. Virol. (1988) 62:3224-3232.
Dorsky et al., "Site-specific mutageneiss of a highly conserved region of the herpes simplex virus type 1 DNA polymerase gene" J. Virol. (1990) 64:1394-1397.
Bernad et al., The highly conserved amino acid sequence motif Tyr-Gly-Thr-Asp-Ser in .alpha.-like DNA polymerases is required by phage .phi.29 DNA polymerase for protein-primed initiation and polymerization Proc. Natl. Acad. Sci. USA (1990) 87:4610-4614.
Gibbs et al., "Identification of amino acids in herpes simplex virus DNA polymerase involved in substrate and drug recognition" Proc. Natl. Acad. Sci. USA (1988) 85:6672-6676.
Blasco et al., "Structural and functional studies on .o slashed.29 DNA polymerase" Chromosoma (1992) 102:S32-S38.
Blasco et al., ".phi.29 DNA polymerase active site" J. Biol. Chem. (1992) 267:19427-19434.
Digard et al., "Specific inhibition of herpes simplex virus DNA polymerase by helical peptides corresponding to the subunit interface" Proc. Natl. Acad. Sci. USA (1995) 92:1456-1460.
Stow, "Sequences at the C-terminus of the herpes simplex virus type 1 UL30 protein are dispensable for DNA polymerase activity but not for viral original-dependent DNA replication" Nucl. Acids Res. (1993) 21:87-92.
Meier et al., Varicella-zoster virus DNA polymerase and major DNA-binding protein genes have overlapping divergent promoters J. Virol. (1993) 67:7573-7581.
Yeung et al., "Differences in the capacity of two herpes simplex virus isolates to spread from eye to brain map to 1610 base pairs of DNA found in the gene for DNA polymerase" Curr. Eye Res. (1991) 10 (Suppl):31-37.
Gen Bank Search, sequence i.d.1, Database: Non-redundant GenBank+EMBL+DDBJ+PDB sequences 245,156 sequesnces; 342,555,758 total letters, 10 pages total.
Gen Bank Search, sequence i.d.2, Database: Non-redundant Genbank CDS translations+PDB+SwissProt+SPudate+PRI 203,899 sequences; 57,984351 total letters, 12 pages total.
Gen Bank Search, sequence i.d.3, Database: Non-redundant GenBank+EMBL+DDBJ+PDB sequences 245,156 sequesnces; 342,555,758 total letters, 10 pages total.
Gen Bank Search, sequence i.d.4, Database: Non-redundant GenBank+EMBL+DDBJ+PDB sequences 245,156 sequesnces; 342,555,758 total letters, 12 pages total.
VanDevanter et al., "Detection and analysis of diverse herpesviral species by consensus primer PCR" J. Clin. Microbiol. (1996) 34:1666-1671.
Gibbs et al., "Polymerization activity of an .alpha.-like DNA polymerase requires a conserved 3'-5' exonuclease active site" Mol. Cell Biol. (1991) 11:4786-4795.Mol. Cell. Biol. (1991) 11:4786-4795.Mol. Cell. Biol. (1991) 11:4786-4785.
Albrecht et al., "Structural organization of the conserved gene block of Herpesvirus saimiri coding for DNA polymerase, glycoprotein B, and major DNA binding protein" Virology (1990) 174:533-542. The related EMBL Sequence Data Library, Heidelberg, BRD, XP002021595, Accession No. P24907 is also enclosed.
Dang et al., "Structural and functional analyses of a yeast mitochondrial ribosomal protein homologous to ribosomal protein S15 of Escherichia coli" Nucleic Acids Research (1990) 18:6895-6901. The related EMBL Sequence Data Library, Heidelberg, BRD, XP002021631, Accession No. S12797 is also enclosed.
Hillier et al., "ya03b03.r2 Homo sapiens cDNA clone 60365 5"EMBL Sequence Data Library, Heidelberg, BRD, XP002021632, Accession No. T39269. 1 page total.
Telford et al., "The DNA sequence of equine herpesvirus 2" J. Mol. Biol. (1995) 249:520-528. The related EMBL Sequence Data Library, Heidelberg, BRD, XP002021596, Accession No. U20824 is also enclosed.
Roizman et al., "New viral footprints in Kaposi's sarcoma" New Engl. J. Med. (1995) 332:1227-1228.
Schalling et al., "A role for a new herpes virus (KSHV) in different forms of Kaposi's sarcoma" Nature Medicine (1995) 1:707-702.
Chang et al. Science vol. 266, Dec. 16, 1994, pp. 1865-1869.
Albrecht et al. J Virol, vol. 66, No. 8, Aug. 1992, pp. 5047-5058.
Bosch Marnix L.
Rose Timothy M.
Strand Kurt
Todaro George J.
Bui Phuong T.
Stucker Jeffrey
University of Washington
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