Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-04-18
1998-05-12
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 912, 435 915, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 536 253, 935 77, 935 78, C12P 1934
Patent
active
057503800
ABSTRACT:
A first polynucleotide is formed with a first particular sequence of nucleotides such as by providing a solid-phase support and adding nucleotides to the support. A second polynucleotide is formed in a similar manner with a second particular sequence of nucleotides which are complementary to the nucleotides in the first polynucleotide at first particular ends of the polynucleotides. The first particular ends may be the 3'-ends of the polynucleotides. The polynucleotides are hybridized or annealed at their 3'-ends to form a base-pair complementary stretch between the first and second polynucleotides. A repair synthesis is then provided in the first and second polynucleotides at second ends (such as the 5'-ends) opposite the first particular ends to provide a double-stranded polynucleotide. The repair synthesis may be monitored by labelling the second end (or 5'-end) of one or both of the nucleotides with radioactivity and then subjecting the nucleotides to an enzymatic reaction.
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Itakura Keiichi
Rossi John J.
City of Hope Research Institute
Marschel Ardin H.
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