DNA polymerase mediated synthesis of double stranded nucleic aci

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 912, 435 915, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 536 253, 935 77, 935 78, C12P 1934

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057503800

ABSTRACT:
A first polynucleotide is formed with a first particular sequence of nucleotides such as by providing a solid-phase support and adding nucleotides to the support. A second polynucleotide is formed in a similar manner with a second particular sequence of nucleotides which are complementary to the nucleotides in the first polynucleotide at first particular ends of the polynucleotides. The first particular ends may be the 3'-ends of the polynucleotides. The polynucleotides are hybridized or annealed at their 3'-ends to form a base-pair complementary stretch between the first and second polynucleotides. A repair synthesis is then provided in the first and second polynucleotides at second ends (such as the 5'-ends) opposite the first particular ends to provide a double-stranded polynucleotide. The repair synthesis may be monitored by labelling the second end (or 5'-end) of one or both of the nucleotides with radioactivity and then subjecting the nucleotides to an enzymatic reaction.

REFERENCES:
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