DNA polymerase III holoenzyme from Escherichia coli

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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536 232, 536 237, 530350, 4353201, 4352523, 43525233, 435325, C12N 912, C12N 1554

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active

056680040

ABSTRACT:
The present invention is directed toward the 5 previously unknown genes, for subunits .delta., .delta.', .chi., .theta., and .psi., of the DNA polymerase III holoenzyme, and toward a unique man-made enzyme containing 5, preferably 6, protein subunits which shows the same activity as the naturally occurring 10 protein subunit DNA polymerase III holoenzyme.

REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
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Kornberg, Arthur, "DNA Replication," W.H. Freeman and Company (San Francisco) 172-78 (1980).
EMBL database entry #ECXERB, Jul. 6, 1989.
Ecoseq database entry #YZPA ECOLI, Oct. 1, 1989.
Studwell et al., "Processive Replication is Contingent on the Exonuclease Subunit of DNA Polymerase III Holoenzyme," The Journal of biological Chemistry 265(2):1171-78 (1990).
Stukenberg, P.T., et al., The Journal of Biological Chemistry 266(17):11328-34 (1991).
Kong, et al., Cell 69:425-37 (1992).
Carter et al., "Molecular Cloning, Sequencing, and Overexpression of the Structural Gene Encoding the Delta Subunit of Escherichia coli DNA Polymerase III Holoenzyme," Journal of Bacteriology 174(21):7013-25 (1992).
EMBL database entry #ECDNAPDPS, Nov. 12, 1992.
EMBL database entry #ECHOLETTA, Nov. 12, 1992.
Maki et al., J. Biol. Chem., 263(14):6547-6554, May 15, 1988.
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Yoshikawa et al., Mol. Gen. Genet. (1987) 209: 481-488.
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Takase et al., J. Bact., 169(12): 5692-5699, Dec. 1987.

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