DNA polymerase having modified nucleotide binding site for DNA s

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 435 911, 4351723, 435 691, 536 231, 530350, C12Q 168, C12P 1934, C12N 900, C07H 2102

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056143656

ABSTRACT:
Modified gene encoding a modified DNA polymerase wherein the modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase.

REFERENCES:
patent: 4707235 (1987-11-01), Englert et al.
patent: 4795699 (1989-01-01), Tabor et al.
patent: 4921794 (1990-05-01), Tabor et al.
patent: 4942130 (1990-07-01), Tabor et al.
patent: 4946786 (1990-08-01), Tabor et al.
patent: 4952496 (1990-08-01), Studier et al.
patent: 4962020 (1990-10-01), Tabor et al.
patent: 4994372 (1991-02-01), Tabor et al.
patent: 4997818 (1991-05-01), McCaffrey et al.
patent: 5001050 (1991-03-01), Blanco et al.
patent: 5079352 (1992-01-01), Gelfand et al.
patent: 5108892 (1992-04-01), Burke et al.
patent: 5122345 (1992-06-01), Tabor et al.
patent: 5145776 (1992-08-01), Tabor et al.
patent: 5173411 (1992-12-01), Tabor et al.
patent: 5198543 (1993-03-01), Blanco et al.
patent: 5210036 (1993-05-01), Comb et al.
patent: 5266466 (1993-11-01), Tabor et al.
patent: 5270179 (1993-12-01), Chatterjee
patent: 5322785 (1994-06-01), Comb et al.
patent: 5352600 (1994-10-01), Gelfand et al.
patent: 5352778 (1994-10-01), Comb et al.
Reha Krantz et al. J. of Virology, 67:60-66, 1993.
Donlin and Johnson, Biochemistry 33: 14908-14917 1994.
Gibbs et al. PNAS 85: 6672-6676 1988.
Larder et al. EMBO 6: 169-175 1987.
Derse et al. JBC 257: 10251-10260 1982.
Prasad et al. PNAS 88: 11363-11367 1991.
Astatke et al., "Deoxynucleoside Triphosphate and Pyrophosphate Binding Sites in the Catalytically Competent Ternary Complex for the Polymerase Reaction Catalyzed by DNA Polymerase I (Klenow Fragment)," J. Biol. Chem. 270:1945-1954 (1995).
Polesky et al., "Identification of Residues Critical for the Polymerase Activity of the Klenow Fragment of DNA Polymerase I from Escherichia coli", J. Biol. Chem. 265(24);14579-14591 (1990).
Prasad et al., "Isolation and Characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase," Proc. Natl. Acad. Sci. USA 88:11363-11367 (1991).
Prober et al., "A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides," Science 238:336-341 (1987).
Reha-Krantz et al., "Mutational Analysis of Bacteriophage T4 DNA Polymerase," from Abstracts for Poster Presentations, presented at a meeting entitled The Fidelity of DNA Synthesis: Structural and Mechanistic Perspectives, Beaufort, North Carolina, Sep. 24-29, 1989.
Reha-Krantz et al., "Bacteriophage T4 DNA Polymerase Mutations that Confer Sensitivity to the PP Analog Phosphonoacetic Acid," J. Virology 67(1):60-66 (1993).
Reha-Krantz et al., "Motif A of Bacteriophage T4 DNA Polymerase: Role in Primer Extension and DNA Replication Fidelity," J. Biol. Chem. 269(8):5635-5643 (1994).
Sanger et al., "DNA sequencing with chain-terminating inhibitors," Proc. Natl. Acad. Sci. USA 74(12):5463-5467 (1977).
Sawaya et al., "Crystal Structure of Rat DAN Polymerase .beta.: Evidence for a Common Polymerase Mechanism," Science 264:1930-1935 (1994).
Smith et al., "The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis," Nucleic Acids Research 13(7):2399-2413 (1985).
Smith et al., "Fluorescence detection in automated DNA sequence analysis," Nature 321:674-679 (1986).
Song et al., "Mutagenesis of the Glu-89 Residue in Human Immunodeficiency Virus Type 1 (HIV-1) and HIV-2 Reverse Transcriptase: Effects on Nucleoside Analog Resistance," J. Virology 66(12):7568-7571 (1992).
Sousa et al., "Crystal struture of bacteriophge T7 RNA polymerase at 3.3 .ANG. resolution," Nature 364:593-599 (1993).
St. Clair et al., "Resistance to ddl and Sensitivity to AZT Indusced by a Mutation in HIV-1 Reverse Transcriptase," Science 253:1557-1559 (1991).
Tabor and Richardson, "Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I," Proc. Natl. Acad. Sci. USA 86:4076-4080 (1989).
Tabor et al., "DNA Sequence Analysis with a Modified Bacteriophage T7 DA Polymerase," `Effect of Pyrophosphorolysis and Metal Ions` J. Biol. Chem. 265(14):8322-8328 (1990).
Tisdale et al., "Rapid in vitro selection of human immunodeficiency virus type 1 resistant to 3'-thiacytidine inhibitors due to a mutation in the YMDD region of reverse transcriptase," Proc. Natl. Acad. Sci. USA 90:5653-5656 (1993).
Toneguzzo et al., "Use of a Chemically Modified T7 DNA Polymerase for Manual and Automated Sequencing of Supercoiled DNA," BioTechniques 6(5):460-469 (1988).
Basu et al., "Identification and Amino Acid Sequence of the Deoxynucleoside Triphosphate Binding Site in Escherichia coli DNA Polymerase," Biochemistry 26:1704-1709 (1987).
Beese et al., "Crystal Structures of the Klenow Fragment of DNA Polymerase I Complexed with Deoxynucleoside Triphosphate and Pyrophosphate," Biochemistry 32:14094-14101 (1993).
Boucher et al., "Ordered Appearance of Zidovudine Resistance Mutations during Treatment of 18 Human Immunodeficiency Virus-Positive Subject," J. Infect. Dis. 165:105-110 (1992).
Carroll et al., "A Mutant of DNA Polymerase I (Klenow Fragment) with Reduced Fidelity," Biochemistry 30:804-813 (1991).
Derse et al., "Characterization of the DNA Polymerases Induced by a Group of Herpes Simplex Virus Type I Variants Selected for Growth in the Presence of Phosphonoformic Acid," J. Biol. Chem. 257(17):10251-10260 (1982).
Donlin and Johnson, "Mutants Affecting Nucleotide Recognition by T7 DNA Polymerase," Biochemistry 33(499:14908-14917 (1994).
Gibbs et al., "Identification of amino acids in herpes simplex virus DNA polymerase involved in substrate and drug recognition," Proc. Natl. Acad. Sci. USA 85:6672-6676 (1988).
Joyce and Seitz, "Function and Structure Relationships in DNA Polymerases," Annu. Rev. Biochem. 63:777-822 (1994).
Kambara et al., "Optimization of Parameters in a DNA Sequenator Using Fluorescence Detection," BioTechnology 6:816-821 (1988).
Kellam et al., "Fifth mutation in human immunodeficiency virus tupe 1 reverse transcriptase contributes to the development of high level resistance to zidovudine," Proc. Natl. Acad. Sci. USA 89:1934-1938 (1992).
Lacey et al., "Biochemical Studies on the Reverse Transcriptase and RNase H Activities from Human Immunodeficiency Virus Strains Resistant to 3'-Azido-3'-deoxythymidine," J. Biol. Chem. 267(22):15789-15794 (1992).
Lacey and Larder, "Mutagenic Study of Codons 74 and 215 of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase, Which are Significant in Nucleoside Analog Resistance," Antimicrobial Agents and Chemotheraphy 68:3421-3424 (1994).
Lacey and Larder, "Novel Mutation (V75T) in Human Immunodeficiency Virus Type 1 Reverse Transcriptase Confers Resistance to 2',3'-Didehydro-2',3'-Dideoxythymidine in Cell Culture," Antimicrobial Agents and Chemotheraphy 38:1428-1432 (1994).
Larder et al., "Related functional domains in virus DNA polymerases," EMBO J. 6(1):169-175 (1987).
Larder, "3'-Azido-3'-Deoxythymidine Resistance Suppressed by a Mutation Conferring Human Immunodeficiency Virus Type 1 Resistance to Nonnucleoside Reverse Transcriptase Inhibitors," Antimicrobial Agents and Chemotheraphy 36:2664-2669 (1992).
Larder et al., "Multiple Mutations in HIV-1 Reverse Transcriptase Confer High-Level Resistance to Zidovudine (AZT)", Science 246:1155-1158 (1989).
Pandey et al., "Role of Lysine 758 of Escherichia coli DNA Polymerase I as Assessed by Site-directed Mutagenesis," J. Biol. Chem. 269(18):13259-13265 (1994).
Pelletier et al., "Structures of Ternary Complexes of Rat DNA Polymerase .beta., a DNA Template-Primer, and ddCTP," Science 254:1891-1903 (1994).
Polesky et al., "Side Chains Involved in Catalysis of the Polymerase Reaction of DNA Polymerase I from Escherichia coli," J. Biol. Chem. 267(12):8417-8428 (1992).
Ito and Braithwaite, "Compilation and Alignment of DNA Polymerase Sequences," Nucleic Acids Research 19:4045-4057 (1991).
Foury and Vanderstraeten, "Yeast mitochondrial DNA mutators wit

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