DNA polymerase having ability to reduce innate selective discrim

Details DNA polymerase having ability to reduce innate selective discrim DNA polymerase having ability to reduce innate selective discrim

1998-09-21

2000-12-26

Stole, Einar

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 6, 435 911, 435 912, C12N 912, C12Q 168, C12P 1934

Patent

active

061657654

ABSTRACT:
The invention relates to genetical modification of DNA polymerase to reduce its innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated florescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.

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