DNA polymerase having 3'-intrinsic editing activity

Details DNA polymerase having 3'-intrinsic editing activity DNA polymerase having 3'-intrinsic editing activity

1996-05-31

1999-12-14

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 915, 435183, 536 231, C12Q 168, C07H 2104, C12P 1934

Patent

active

060015667

ABSTRACT:
The invention is directed to the use of RNA or DNA polymerases having 3'-intrinsic editing activity (3'-IEA) in the presence or absence of a deactivating agent to remove a protecting group from the 3'-position of oligo- polyribo- or deoxyribonnucleotides. The use is connected with the incorporation of dNTPs into DNA templates in order to determine the concentration and/or sequence of said templates. In particular the use is concerned with an improved non gel-based sequencing method.

REFERENCES:
Canard et al., DNA polymerase fluorescent substrates with reversible 3' tags, Gene, vol. 148, pp. 1-6, Oct. 1994.
Murray et al., The determination of the sequences present in the shadow bands of a dinucleotide repeat PCR, Nucleic Acids Research, vol. 21(10), pp. 2395-2398, 1993.

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