Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-05-14
1997-01-07
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 694, 435 9121, 435 9131, 4351723, 4352402, 4353201, 536 231, 536 241, 536 245, 514 44, C12N 516, C12N 1518, C12N 1585
Patent
active
055916015
ABSTRACT:
The present invention relates to a gene expression system in eukaryotic cells. In particular, it relates to a self-initiating and self-sustaining gene expression system utilizing an RNA polymerase prebound to a DNA construct before the introduction of the complex into cells. This system is capable of functioning in the cell cytoplasm without integration into the genome of host cells, and does not require host cell factors for initiation and maintenance of gene expression. Therefore, the invention has a wide range of applications in in vitro and in vivo gene expression, including gene therapy in resting cells.
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Dubendorff and Studier, "Creation of a T7 Autogene: Cloning and Expression of the Gene for Bacteriophage T7 RNA Polymerase under Control of its Cognate Promoter", J Mol Biol 219:61-68 (1991).
Chen Xiaozhuo
Li Yunsheng
Wagner Thomas E.
LeGuyader John L.
Ohio University Edison Animal Biotechnology Institute
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