DNA polymerase blends and uses thereof

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S183000, C435S069700, C435S091100, C530S350000

Reexamination Certificate

active

07960157

ABSTRACT:
The present invention discloses novel blends of chimeric and non-chimeric thermostable DNA polymerases for use in PCR, DNA sequencing and mutagenesis protocols. The invention allows for PCR reactions with shorter extension times that will facilitate PCR amplification of genomic DNA templates and improve the efficacy of long PCR.

REFERENCES:
patent: 5489523 (1996-02-01), Mathur
patent: 5556772 (1996-09-01), Sorge et al.
patent: 5834285 (1998-11-01), Comb et al.
patent: 5972603 (1999-10-01), Bedford et al.
patent: 6228628 (2001-05-01), Gelfand et al.
patent: 2002/0119461 (2002-08-01), Chatterjee
patent: 2002/0164618 (2002-11-01), Callen et al.
patent: 11089588 (1999-04-01), None
patent: 2001269188 (2001-10-01), None
patent: WO9729209 (1997-08-01), None
patent: WO0118213 (2001-03-01), None
patent: 0132887 (2001-05-01), None
patent: WO0161015 (2001-08-01), None
patent: WO01/92501 (2001-12-01), None
patent: 0204022 (2002-01-01), None
patent: WO0234936 (2002-05-01), None
patent: 03060144 (2003-07-01), None
patent: 2004038007 (2004-05-01), None
patent: 2004087868 (2004-10-01), None
Ngo et al. in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
Komori et al., Protein Engineering, vol. 13, No. 1, pp. 41-47, 2000.
International Search Report (PCT/US03/40421).
Bedford, et. al., PNAS (1997); v. 94, pp. 479-484.
Lim, et. al., J. Biol. Chem, (1999), v. 274(53): 38197-38203.
Motz, M., et al., J. Biol. Chem, (May 3, 2002); 277 (18); 16179-88.
Barnes, Wayne M., “PCR amplification of up to 35-kb DNA with high fidelity and high yield from γ bacteriophage templates,” Proc. Natl. Acad. Sci. USA, Mar. 1994, vol. 91, pp. 2216-2220.
Pavlov, et al., “Helix-hairpin-helix motifs confer salt resistance and processivity on chimeric DNA polymerases,” Proc. Natl. Acad. Sci. USA, Oct. 2002, vol. 99, No. 21, pp. 13510-13515.
Shimazaki, et al., “Over-expression of human DNA polymerase lambda inE. coliand characterization of the recombinant enzyme,” Genes to Cells, Jul. 2002, vol. 7, pp. 639-651.
Supplementary Partial European Search Report, EP03797055, May 24, 2006.
Fogg, Mark J., et al., “Structural Basis for Uracil Recognition by Archaeal Family B DNA Polymerases”, Nature Structural Biology, (2002) vol. 9, No. 12, pp. 922-927.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

DNA polymerase blends and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA polymerase blends and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA polymerase blends and uses thereof will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2694722

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.