DNA molecules encoding platelet-derived growth factor B chain an

Details DNA molecules encoding platelet-derived growth factor B chain an DNA molecules encoding platelet-derived growth factor B chain an

1990-12-13

1993-12-21

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525233, 4351721, 536 235, 530399, C12P 2106, C12N 122, C07H 1512

Patent

active

052720640

ABSTRACT:
Novel platelet-derived growth factor (PDGF) analogs are provided in accordance with the present invention. Also provided is a method for the production of homogeneous quantities of these novel analogs. The novel analogs of the present invention, when refolded, have substantially the same biological activity as naturally occurring PDGF B.sub.109. The method of the present invention employs the use of a stop codon on the c-sis gene, or other coding sequence for a precursor protein of PDGF B.sub.109, or analogs thereof, at a position corresponding to a location from about amino acid 111 to about amino acid 160. The method of the present invention results in the production of relatively large homogeneous quantities of recombinant PDGF B analogs from high expression host cells, such as E. coli.

REFERENCES:
patent: 4479896 (1984-10-01), Antoniades
patent: 4769328 (1988-09-01), Murray et al.
patent: 4801542 (1989-01-01), Murray et al.
patent: 4845075 (1989-04-01), Murray et al.

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