DNA molecules encoding L-glutamate-gated chloride channels...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S320100, C435S325000, C435S252300, C435S254110, C435S254200, C536S023500, C530S350000

Reexamination Certificate

active

10239588

ABSTRACT:
The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encodeRhipicephalus sanguineusglutamate gated chloride channels. The present invention also relates to recombinant vectors and recombinant hosts which contain a DNA fragment encodingR. sanguineusglutamate gated chloride channels, substantially purified forms of associatedR. sanguineusglutamate gated chloride channels and recombinant membrane fractions comprising these proteins, associated mutant proteins, and methods associated with identifying compounds which modulate associatedRhipicephalus sanguineusglutamate gated chloride channels, which will be useful as insecticides.

REFERENCES:
patent: 5693492 (1997-12-01), Cully et al.
patent: 6358701 (2002-03-01), Warmke et al.
patent: WO-99/07828 (1999-02-01), None
patent: WO-01/30849 (2001-05-01), None
patent: WO-01/74899 (2001-10-01), None
patent: WO-01/96396 (2001-12-01), None
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Arena et al. ‘Expression of a glutamate-activated chloride current inXenopus oocytesinjected withCaenorhabditis elegansRNA: evidence for modulation by avermectin’, Molecular Brain Research 15: 339-348 (1992).
Database NCBI. Nov. 18, 1997. Accession No. AJ002232, Semenov, et al. Direct submission.
Supplementary European Search Report issued May 4, 2004 in European Patent Application No. 01922781.8.
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Database EMBL. Aug. 17, 1998. “Lucilia cuprina glutamate gated chloride chanel mRNA, Compete cds.” retrieved from EBI Database accession No. AF081674.
Database EMBL. Jun. 7, 1999. “Cat flea glutamate gated chloride channel CfGluCl-1 cDNA.” retrived from EBI Database accession No. AAX24372 (Applicants believe this sequence is included in International Patent Appn. No. PCT/US98/16613, which published as International Pub. No. WO 99/07828 and is cited From SB/08A submitted concurrently herewith).

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