DNA molecules and vectors regarding clavulanic acid biosynthesis

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

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4351723, 4352523, 43525233, 43525235, 4353201, 536 232, 536 237, C12N 1574, C07H 2104

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057598312

ABSTRACT:
DNA sequences obtained from S. clavuligerus, recombinant vectors incorporating such sequences and hosts transformed with such vectors are disclosed. The DNA comprises one or more genes encoding one or more enzymes involved in the biosynthesis of clavulanic acid and may be used to improve the yield of clavulanic acid produced by clavulanic acid-producing organisms such as S. clavuligerus ATCC 27064.

REFERENCES:
Bailey et al. Biotechnology 2:808-811 (1984).
Romero et al. Appl. Microbiol. Biotechnol. 27:510-519 (1988).
F. Malpartida and D.A. Hopwood, "Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic . . . , " Nature, vol. 309, 462-4 (31 May 1984).
J. Romero et al., "Dissociation of cephamycin and clavulanic acid biosynthesis . . . ," Appl. Microbiol. Biotechnol., 1984, 20:318-325.

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