Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-04-14
1996-05-28
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352402, 4352523, 43525233, 43525411, 4353201, 530326, 536 232, C07K 708, C12N 948, C12N 1557, C12N 1563
Patent
active
055210813
ABSTRACT:
The invention concerns DNA encoding prolylendopeptidase, hybrid vectors containing such DNA, transformed hosts capable of expressing prolylendopeptidase, a process for the production of prolylendopeptidase including the steps of: culturing a host organism transformed with an expression vector including a DNA coding for prolylendopeptidase and optionally, recovering the produced prolylendopeptidase; and a process for the production of a C-terminal amidated peptide from two precursors, including the steps of: placing the two precursors in contact with a prolylendopeptidase in a medium to convert the precursor peptides to the C-terminal amidated peptide, and recovering the resulting C-terminal amidated peptide.
REFERENCES:
patent: 4865974 (1989-09-01), Ben-Bassat et al.
Gerald et al., Biochim. Biophys. Acta 866:1-14 (1986).
Young et al., Science 222:778-782 (1983).
Diefenthal et al., Appl. Microbiol. Biotechnol. 40:90-97 (1993).
Sambrook et al., Molecular Cloning, A Laboratory Manual, 2nd Edition, Cold Spring Harbor Lab. Press, Cold Spring Harbor, NY, 1989, pp. 11-1 to 11-19.
1992 Sigma Chemical Co. Catalog, Sigma Chemical Co., St. Louis, MO, 1992, pp. 395, 855-857, 1756.
Yoshimoto, T. et al.; J. Biol. Chem. 255: 4786-4792 (1980).
Nakamura, S. et al.; Chem. Abstracts 113: 110485w (1990).
Garel, M.-C. et al.; J. Biol. Chem 264: 18966-18972 (1989).
Chevallier, S. et al.: Characterization of a Prolyl Endopeptidase from Flavobacterium meningosepticum. J. Biol. Chemistry 267: 8192-8199 (1992).
Diefenthal, T.: F. meningosepticum F1PEP1 Gene for Proline-Specific Endopeptidase. EMBL DNA Database, Entry FMF1PEP1, Accession Number X63674, (1992).
DeBoer, H. A. et al.: The Tac Promoter: A Functional Hybrid Derived from the TRP and LAC Promoters. Proc. Natl. Acad. Sci. USA 80:21-25 (1983).
Rennex, D. et al.: cDNA Cloning of Porcine Brain Prolyl Endopeptidase and Identification of the Active--Site Seryl Residue. Biochemistry 30: 2195-2203 (1991).
Yoshimoto, T. et al.: Prolyl Endopeptidase from Flavobacterium Meningosepticum: Cloning and Sequencing of the Enzyme Gene. J. Biochem. 110:873-878 (1991).
Inaoka Tetsuya
Kokubo Toshio
Tsuru Daisuke
Yoshimoto Tadashi
Ciba-Geigy (Japan) Limited
Grimes Eric
Wax Robert A.
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