DNA microwell device and method

Details DNA microwell device and method DNA microwell device and method

1996-09-26

1998-08-18

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

422 8205, 422 8207, 422 8209, 435 911, G01N 2129, G01N 2164, G01N 2127, C12P 1934

Patent

active

057957487

ABSTRACT:
An apparatus for carrying out a homogeneous nucleic acid amplification and nucleic acid assay on a liquid biological sample comprises a sample well and an optical window element which is received in the sample well. Opposed, spaced-apart surfaces of the optical window element and sample well define a capillary chamber into which a liquid biological sample is drawn by capillary force. By spreading the liquid biological sample into a thin film within the capillary chamber, head space is eliminated, heat transfer to the sample is maximized, and a large optical target is achieved to facilitate the detection step of the assay. The disclosed apparatus is particularly suited for use with homogeneous nucleic acid amplification and fluorescence polarization assays, but can also be used in connection with other types of biological and chemical processes.

REFERENCES:
patent: 5047206 (1991-09-01), Dombrowski
patent: 5229297 (1993-07-01), Schnipelsky
patent: 5429804 (1995-07-01), Sayles
patent: 5587128 (1996-12-01), Wilding
patent: 5593867 (1997-01-01), Walker

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