DNA measuring method

Details DNA measuring method DNA measuring method

1992-09-09

1994-10-18

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

2041828, 935 77, 935 78, 436 94, C12Q 170

Patent

active

053567765

ABSTRACT:
DNA molecule length can be measured with high precision and efficiency by 1) using such means as electrophoresis gel migration to orient a DNA molecule having a fluorescence label at both its termini into a straight line by its passing through a migration path having in a portion of it an area not more than several micrometers in diameter, detecting the fluorescence label at both the termini at a predetermined location and measuring the interval between the detection of the fluorescence coming from one terminus and that of the fluorescence from the other or by 2) a DNA molecule bound to a fluorescence label at one terminus and to a particle at the other being led as a whole by such means as electric field application into an aperture smaller in diameter than the particle, leaving the particle fixed at the mouth of the aperture to stretch the DNA molecule and detecting the fluorescence position to measure the distance between the bound particle and the bound fluorescence label.

REFERENCES:
patent: 5079169 (1992-01-01), Chu
Zhang et al Clin Chem (Sep. 1991) 37:1492-1496.
Smith et al Biopolymers (1990) 29:1167-1173.
Urdea et al Nucl Acids Res (1988) 16:4937-4955.

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