DNA markers to detect cancer cells expressing a mutator phenotyp

Details DNA markers to detect cancer cells expressing a mutator phenotyp DNA markers to detect cancer cells expressing a mutator phenotyp

1996-06-10

1998-06-30

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 243, C12P 1934

Patent

active

057736499

ABSTRACT:
The present invention relates to a method of diagnosis or prognosis of tumor cells in a patient displaying a mutator phenotype in the tumor which comprises the steps of: a) obtaining a genomic DNA sample of the tumor; b) obtaining a genomic DNA sample of a tumor-free tissue of the patient; subjecting the DNA samples of steps a) and b) to amplification using primers which are flanking repeat pattern affected by a mutator phenotype or cold inter-Alu PCR followed by hybridization with a probe corresponding to an instability prone locus; d) subjecting the amplified fragments of step c) to electrophoretic fractionation on a polyacrylamide gel to determine the presence of a variation in band profile between tumor and tumor-free DNA indicating a genomic instability associated with a mutator phenotype; and e) employing means for comparing the allelic status of at least one instability prone locus in the DNA samples of step c).

REFERENCES:
Speicher, M.R., 1995, Oncology Res., 7:267-275.
Kolodner, R.D. et al., 1995, TIBS, 20:397-401.
Sinnett, D. et al., Genomics, 7:331-334.
Zietkiewicz, E. et al., 1992, Proc. Natl. Acad. Sci. USA, 89:8448-8451.
Peinado, M.A. et al., 1992, Proc. Natl. Acad. Sci. USA, 89:10065-10069.

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