DNA ligase mutants

Details DNA ligase mutants DNA ligase mutants

2011-04-19

2011-04-19

Hutson, Richard G (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S183000, C435S091100, C530S350000

Reexamination Certificate

active

07927853

ABSTRACT:
It is intended to obtain DNA ligase improved in binding ability and reactivity with DNA, particularly thermostable DNA ligase improved in binding ability and reactivity with DNA. The present invention provides a DNA ligase mutant improved in binding ability and reactivity with DNA, which is obtained by partially or completely deleting a C-terminal helix portion of DNA ligase. Particularly, the mutant is derived from a thermostable bacterium.

REFERENCES:
patent: 5506137 (1996-04-01), Mathur et al.
patent: 6280998 (2001-08-01), Mathur et al.
patent: 6576453 (2003-06-01), Barany et al.
Ngo et al. In the Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
Jeon, Sung-Jong, “Development of Extremely Thermostable DNA Ligase for Genetic Diagnosis”, AIST Today, vol. 11, Winter 2004, 1 page Abstract, (4 pages in Japanese).

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