DNA ligase assay

Details DNA ligase assay DNA ligase assay

1998-05-27

1999-11-02

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 8, 435 9152, C12Q 168, C12Q 100, C12Q 166, C12P 1934

Patent

active

059768061

ABSTRACT:
A quantitative and functional DNA ligase assay is disclosed. The assay involves the disruption, using restriction enzymes, of a plasmid containing a reporter gene, followed by a ligation step performed in the presence of the biological sample being assayed for DNA ligase activity. The ligation reaction products are then subjected to a coupled transcription-translation reaction, and the extent of DNA ligation is then quantified.

REFERENCES:
Engler et al., "DNA Ligases," in The Enzymes, vol. XV Nucleic Acids Part B, third edition, Paul D. Boyer, ed., Academic Press: New York, 1982, pp. 3-29.
Barker et al., Mol. Gen. Genet. 200:458-462 (1985).
Abravaya et al., Nuc. Acid. Res. 23(4):675-682 (1995).

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