Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1978-04-21
1981-04-28
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435172, 435317, 435849, C12P 1934, C12N 1500, C12R 119
Patent
active
042647312
ABSTRACT:
A selected portion of DNA molecules having reactant ends which are capable of being joined in a ligase catalyzed reaction are pretreated so a to remove the 5'-terminal phosphate groups. Such a treatment reduces the frequency of joining an undersired combination and enhances the frequency of joining the desired combination.
REFERENCES:
Molecular Cloning of Recombinant DNA, Miami Winter Symposium, vol. 13, pp. 35-56 (1977).
Tanenholtz Alvin E.
The Regents of the University of California
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