DNA isolation method

Details DNA isolation method DNA isolation method

2005-02-08

2005-02-08

Ketter, James (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S306100, C435S317100

Reexamination Certificate

active

06852851

ABSTRACT:
Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membrane and at least some of the nuclear membranes, and introducing the lysate into micro-channels of a microfabricated apparatus in which each of the micro-channels is provided with a barrier disposed in the channel to impede the passage or flow of DNA and cell nuclei each while allowing the passage of liquid through the micro-channel so that a mesh comprising DNA is formed in the channel.

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