Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism
Patent
1990-12-26
1993-04-20
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Treating animal or plant material or micro-organism
435259, 435820, 435 91, 536 231, C12N 108, C12N 106, C12P 1934
Patent
active
052042464
ABSTRACT:
A novel method for the isolation of high molecular weight DNA from plants, yeast and bacteria using xanthate-forming compounds such as sodium/potassium ethyl xanthogenate is disclosed. The procedure does not require deproteination and yields clean DNA that is suitable for both PCR and Southern blotting. It can be utilized on a small scale without homogenizing the tissue. These features also facilitate automated screening of plant tissue samples, one of the labor-intensive techniques in molecular biology. This method is also adaptable for use in the field.
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Lilling Herbert J.
Pearlmutter Nina L.
Pioneer Hi-Bred International , Inc.
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