DNA helicase

Details DNA helicase DNA helicase

2005-09-06

2005-09-06

Hutson, Richard (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S194000, C435S183000, C435S195000, C530S350000

Reexamination Certificate

active

06939699

ABSTRACT:
The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such asEscherichia coliin the usual manner to create a transformant, and culturing the transformant.

REFERENCES:
Ngo et al., Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox, in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
Kawarabayasi et al., Database PIR 78, Accession No. C71231, Aug. 1998.
EP Search Report dated Sep. 1, 2003 for Ref. 303025EP/JND/AV, App. No. 03251213.9-1521-.
Database EM_PRO Online! EMBL; Jun. 17, 1998 Director-General of Giotechnology Center et al.: “Pyrococcus horikoshi OT3 genomic DNA, 1-287000 nt. Position 1/7” retried from EBI Database accession no. AP000001 XP002251724 *abstract*.
Database Swall Online! Aug. 1, 1998 Kawarabayasi et al.: Hypothetical protein PH0109 retried from EBI Database accession No. 057849 XP002251725 *the whole document*.
Kawarabayasi et al.: “Complete sequence and gene organization of the genome of a hyperthermophilic Archaebacterium, Pyrococcus horikoshii OT3” DNA Research, vol. 5, 1998, p.p. 55-76, XP002948248 “p.p. 55-56, left-hand column, paragraph 2* *p. 74, line 11”.
Kyrpides et al.: “Transcription in Archaea” Prodeedings of the National Academy of Sciences, vol. 96, Jul. 1999, p.p. 8545-8550, XP002251723 “table 3”.
Database Swall Online! May 1, 2000 Heilig, R.: “DNA helicase related protein” retrieved from EBI Database accession No. Q9V2G5 XP002251726 *the whole document*.
Database GSP Online! EMBL; May 14, 2001 Hogrefe et al.: “Recombinant P. furiosus helicase 7” retrieved from EBI Database accession No. AAB62030 XP002251727 & WO 01 09347 A (Stratagene) Feb. 8, 2001 *the whole document*.

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