DNA having genetic information of L-.alpha.-glycerophosphate oxi

Details DNA having genetic information of L-.alpha.-glycerophosphate oxi DNA having genetic information of L-.alpha.-glycerophosphate oxi

1988-12-12

1990-10-02

Schwartz, Richard A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4352523, 43525231, 43525233, 435 691, 935 69, 935 73, 935 74, 935 60, 935 14, C12N 1553, C12N 121

Patent

active

049608770

ABSTRACT:
DNA having a base sequence encoding a specified amino acid sequence of a polypeptide constituting an L-.alpha.-glycerophosphate oxidase (GPO) is disclosed. A polypeptide having the specified amino acid sequence can be produced by inserting the DNA into a plasmid, culturing a transformant produced by introducing this plasmid into a host microorganism which is selected from microorganisms belonging to Escherichia coli, Bacillus subtilis, or Saccharomyces cerevisiae. The GPO is useful for the quantitative analysis of triglyceride, glycerine, etc., and the determination of lipase activity, when used in combination with other enzymes such as lipase, glycerol kinase, or the like, or with an ATP reagent.

REFERENCES:
patent: 4275161 (1981-06-01), Misaki et al.
patent: 4463095 (1984-07-01), Emi et al.
The Journal of Biological Chemistry, vol. 254, pp. 2710-2715 (1979).
The Journal of Biological Chemistry, vol. 261, pp. 14396-14407 (1986).
Search Report for French Patent Application 88 16486.
CA 110: 19322t, A Genetic Analysis of the .alpha. Glycerophosphate Oxidase Locus in D. Melanogaster, Davis et al.

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