Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1981-10-19
1983-11-29
Tanenholtz, Alvin E.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 68, 435 70, 435 91, 435172, 435253, 435317, C07N 1512, C12P 2100, C12P 2102, C12P 1934, C12N 1500, C12N 120, C12N 100
Patent
active
044181949
ABSTRACT:
The present invention relates to novel, broad bacterial host range small plasmid deoxyribonucleic acid rings which serve as cloning vehicles for DNA fragments, particularly those separated from other plasmid rings or from chromosones, recombined with the small plasmid rings and to the processes for recombining the plasmid rings and to processes for transferring them between host bacteria. In particular, the present invention relates to the aggregate plasmid ring RP1/pRO1600, to pRO1600 and plasmid ring derivatives thereof, particularly including pRO1601; pRO1613 and pRO1614, all of which are carried for reference purposes in Pseudomonas aeruginosa ATCC 15692 (also known as strain PAO1c) and are on deposit at the Northern Regional Research Laboratories (NRRL) of the United States Department of Agriculture at Peoria, Ill. The plasmid ring RP1 (also known as R1822) is deposited in Pseudomonas aeruginosa NRRL-B-12123 (and is a known plasmid ring). The pRO1600 portion of the aggregate is a new plasmid ring. The novel small plasmid rings are particularly useful for recombinant genetic manipulation wherein the DNA fragments are introduced into the plasmid rings to produce useful, cloneable characteristics in the host bacterium, particularly chemical generating characteristics.
REFERENCES:
Bethesda Research Laboratories Restriction Enzyme Reference Chart (1981).
Grinsted et al.; Plasmid 1, 34 (1977).
Hansen et al.; J. Bact. 135, 227 (1978).
Martinell J.
McLeod Ian C.
Microlife Technics, Inc.
Tanenholtz Alvin E.
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