DNA fragments containing alkaline cellulase gene, recombinant pl

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435320, 435209, 4351723, 43525231, 4352523, 536 27, 935 14, 935 27, 935 56, 935 60, 935 72, 935 73, C12N 942, C12N 700, C12N 1500, C07H 1512

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049045997

ABSTRACT:
DNA frangments containing an alkaline cellulase K gene, which comprise about 4.0, about 2.4 and about 1.9 kilo base pairs, respectively, each having a specific restruction map, recombinant plasmids containing any of these DNA fragments, and recombinant microorganisms harboring any of said plasmids.
The alkaline cellulase K gene is derived from a bacterial strain which belongs to the genus Bacillus and is alkalophilic, that is, capable of revealing an optimum growth in an alkaline pH region.

REFERENCES:
Fukumori, F. et al., J. Gen. Micro., 132:2329-2335, 1986.
Nakamura et al., Eur. J. Biochem, 164(2):317-20, 1987.
Biotechnology Advances, vol. 2, No. 2, 1984, pp. 201-216, Pergamon Press, Ltd, Oxford, GB; V. L. Seligy, Jr. et al.: "Applications of Recombinant DNA Technology to the Pulp and Paper Industry".
Agriculture and Biological Chemistry, vol. 50, No. 1, Jan. 1986, pp. 233-237, Tokyo, JP; Y. Koide et al.: "Molecular Cloning of a Cellulase Gene from Bacillus subtilis and its Expression in Escherichia coli".
Journal of Bacteriology, vol. 158, No. 2, May 1984, pp. 503-506, American Society for Microbiology; S. Nobuhiro et al.: "Molecular Cloning and Expression of Cellulase Genes of Alkalophilic Bacillus sp. strain N-4 in Escherichia coli".
Journal of Bacteriology, vol. 165, No. 2, Feb. 1986, pp. 612-619, American Society for Microbiology; L. M. Robson et al.: "Cloning of the Bacillus subtilis DLG beta-1,4-Glucanase Gene and Its Expression in Escherichia coli and B. subtilis".
Chemical Abstracts, vol. 108, 1988, p. 166, Abstract No. 32738m, Columbus, Ohio, US; T. Kudo et al.: "Alkaline Cellulase Genes from Alkalophilic Bacillus", & Iden 1987, 41(8), 26-9, abstract only.

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