DNA fragments coding for a bacteriophage-resistant mechanism

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4352523, 4353201, 536 237, 536 2432, 935 29, C12N 1531, C12N 121, C12N 1574, C07H 2104

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056291825

ABSTRACT:
The invention relates to DNA fragments encoding an Abi-type mechanism of resistance to bacteriophages, which fragments are capable of being obtained by cloning of chromosomal or plasmid DNA of a bacteriophage-resistant lactic acid bacterial strain, as well as to a polypeptide involved in an Abi-type resistance mechanism, encoded by one of the said fragments. The invention also encompasses recombinant vectors and transformed bacterial strains comprising the said DNA fragments.

REFERENCES:
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Journal of Bacteriology, vol. 172, No. 8, Aug. 1990, D.A. Romero et al.: "Characterization of Insertion Sequence IS946, AN Iso-ISS1 Element, ISO; ATED From the Conjugative Lactoccoccal Plasmid pTR2030", pp. 4151-4160.
Applied and Environmental Microbiology, vol. 56, No. 7, Jul. 1990, C. Hill et al.: "Nucleotide Sequence and Distribution of the pTR2030 Resistance Determinant (hsp) which Aborts Bacteriophage Infection in Lactococci", pp. 2255-2258.
Applied and Environmental Microbiology, vol. 55, No. 7, Jul. 1989, C. Hill et al.: "Localization Cloning and Expression of Genetic Determinants for Bacteriophage Resistance (hsp) From the Conjugative Plasmid pTR2030", pp. 1684-1689.
Biochimie, vol. 70, 1988 (Paris, FR), M.E. Sanders: "Phage Resistance in Lactic Acid Bacteria", pp. 411-421.
Coffey et al "Identification and characterization of a plasmid . . . " Neth. Milk Dairy J. 43:229-244 (1989).
Coffey et al "Cloning and characterization of the determinant . . . " J. Gen. Microbiol. 137:1355-1362 (Jun. 1991).
Gautier et al, "Plasmid--Determined Systems for . . . Abortive Infection in Streptococcus cremoris", Appl, Environ. Microbiol. 53(5):923-927 (May 1987).
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Chopin et al "Two Plasmid--Determined . . . Systems in Streptococcus lactis" Plasmid 11:260-263 (May 1984).
Sing et al "Plasmid--Induced Abortive Infection in Lactococci : A Review" J. Dairy Science 73:2239-2251 (Sep. 1990).

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