DNA fragment specific to cytoplasmic male sterile pepper and...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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Details

C800S278000, C800S288000, C800S306000, C800S317100, C800S317300, C800S317400, C435S069800, C536S023600

Reexamination Certificate

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07728194

ABSTRACT:
The present invention relates to a DNA fragment specific to a cytoplasmic male sterile pepper comprising a polynucleotide of SEQ ID NO: 1, a candidate polynucleotide (named orf456) associated with cytoplasmic male sterile pepper consisting of a 223rdto 678thnucleic acid of SEQ ID NO:1, and a polynucleotide of SEQ ID NO: 2. The DNA fragment specific to cytoplasmic male sterile pepper comprising a polynucleotide of SEQ ID NOs: 1 or 2 can be used for identifying cytoplasmic type between male sterile and male fertile pepper by the PCR method. In addition, hybrid pepper breeders/seed companies could detect impurities of the maintainer line within the CMS line, and by ensuring purity of the CMS line, a major source of contamination of the hybrid seeds is removed leading to obvious benefits for the seed industry and farmers.

REFERENCES:
patent: 6479735 (2002-11-01), Araya et al.
Kim et al. Journal of the Korean Society for Horticultural Science 42(2): 121-127 (2001).
Araya et al. Accession No. AR254351, SEQ ID No:5 of US 6,479,735 (2002).

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