DNA fragment sizing and sorting by laser-induced fluorescence

Details DNA fragment sizing and sorting by laser-induced fluorescence DNA fragment sizing and sorting by laser-induced fluorescence

1995-06-05

1996-09-24

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436 52, 436 63, C12Q 168, G01N 33483, G01N 3508

Patent

active

055589987

ABSTRACT:
A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

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