DNA fragment encoding a polypeptide having nitrile hydratase act

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435227, 4353201, 4352523, 435325, 43525411, 4351723, 536 23, C12P 1302, C12N 100, C12N 500, C12N 978, C12N 1555, C12N 1563

Patent

active

057311765

ABSTRACT:
The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.

REFERENCES:
Nagasawa et al., "Nitrile Hydratase-Catalyzed Production of Nicotinamide from 3-Cyanopyridine in Rhodococcus rhodocrous J1" Appl. Env. Microbiol. (1988) 54(7):1766-1769.
Nishiyama et al., "Cloning and Characterization of genes Responsible for the Metabolism of Nitrile Compounds from Pseudomonas chlororaphis B23" J. Bacteriol., (1991) 173(8):2465-2472.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

DNA fragment encoding a polypeptide having nitrile hydratase act does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA fragment encoding a polypeptide having nitrile hydratase act, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA fragment encoding a polypeptide having nitrile hydratase act will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2288022

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.