Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound
Patent
1993-03-09
1998-03-24
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing nitrogen-containing organic compound
435227, 4353201, 4352523, 435325, 43525411, 4351723, 536 23, C12P 1302, C12N 100, C12N 500, C12N 978, C12N 1555, C12N 1563
Patent
active
057311765
ABSTRACT:
The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.
REFERENCES:
Nagasawa et al., "Nitrile Hydratase-Catalyzed Production of Nicotinamide from 3-Cyanopyridine in Rhodococcus rhodocrous J1" Appl. Env. Microbiol. (1988) 54(7):1766-1769.
Nishiyama et al., "Cloning and Characterization of genes Responsible for the Metabolism of Nitrile Compounds from Pseudomonas chlororaphis B23" J. Bacteriol., (1991) 173(8):2465-2472.
Beppu Teruhiko
Horinouch Sueharu
Nagasawa Toru
Nishiyama Makoto
Yamada Hideaki
Beppu Teruhiko
Bugaisky Gabriele E.
Nitto Chemical Industry Co. Ltd.
Wax Robert A.
Yamada Hideaki
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