Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-05
1997-09-09
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4353201, 536 232, 536 241, C12N 918, C12N 1500, C07H 2104
Patent
active
056655843
ABSTRACT:
The present invention relates to a DNA fragment of 3,563 base pairs containing a gene coding for tannase and derived from a microorganism belonging to the genus Aspergillus, with the following restriction enzyme map: ##STR1## B: Bam HI, H: Hind III, K: Kpn I, S: Sal I, X: Xba I; a DNA fragment containing a tannase gene coding for the amino acid sequence of (SEQ ID NO:4); a recombinant plasmid comprising the DNA fragment containing the tannase gene inserted into a plasmid vector; a process for producing tannase, comprising culturing a microorganism belonging to the genus Aspergillus capable of producing tannase in medium with the recombinant plasmid, and recovering tannase from the culture; and a promoter represented by the nucleotide sequence of (SEQ ID NO:1). Tannase can be efficiently produced according to the present invention.
REFERENCES:
patent: 3812266 (1974-05-01), Sanderson et al.
patent: 4426448 (1984-01-01), Okamura et al.
Hatamoto Osamu
Mizusawa Kiyoshi
Nakano Eiichi
Watarai Teruo
Hobbs Lisa J.
Kikkoman Corporation
Noda Institutute For Scientific Research
Wax Robert A.
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