DNA fragment coding for mercuric reductase of thiobacillus, and

Chemistry: molecular biology and microbiology – Vector – per se

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536 232, 435189, 4351721, 4351723, C12N 1500, C12N 902

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active

053488881

ABSTRACT:
Microorganisms of the genus Thiobacillus find utility in the mining industry in such applications as bacterial leaching, the treatment of mine drainage water and the deiron in hydrometallurgy process. This invention discloses a DNA fragment which codes for a mercuric reductase in microorganisms of the genus Thiobacillus and is useful in constructing a new strain of Thiobacillus ferrooxidans capable of fast growth. A recombinant plasmid incorporating the DNA fragment, a shuttle vector plasmid incorporating the DNA fragment, host cells and a method of transforming the host cells are also disclosed.

REFERENCES:
Shinatori et al. J. Bact., Jun. 1989, vol. 171, No. 6, pp. 3458-3464.
Kusano et al. J. Bact., May 1990, vol. 172, No. 5, pp. 2688-2692.
Olson et al., J. Bact., Sep. 1982, vol. 151, No. 3, pp. 1230-1236.
Booth et al., J. Gen. Microb., 1984, vol. 130, pp. 725-730.
Nishikawa et al., Agric. Biol. Chem., vol. 49, No. 5, pp. 1513-1515.

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