DNA for use in a simple method for detecting inhibitors of retro

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

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435 6, 4352523, 4352402, 4353201, 536 231, C12N 1500, C07H 1700

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054628733

ABSTRACT:
The present invention relates, in general, to a DNA segment. In particular, the present invention relates to a DNA segment comprising a selectable marker gene, a DNA segment comprising a selectable marker gene inserted into a retrotransposon, cells containing these DNA segments, and methods of using these DNA segments and cells.

REFERENCES:
Heidmann, Odile and Thierry, Retrotransposition of a Mouse IAP Sequence Tagged with an Indicator Gene, Cell, Jan. 11, 1991, vol. 84, pp. 159-170.
Garfinkel, D. et al., Transposon Tagging Using Ty Elements in Yeast, Genetics, Sep., 1988, vol. 120, pp. 95-108.
Boeke, J. D. et al, A General Method for the Chromosomal Amplification of Genes In Yeast, Science, vol. 239, pp. 280-282.
Boeke, J. D. et al, Ty Elements Transpose through an RNA Intermediate, Cell, Mar. 1985, vol. 40, pp. 491-500.
Heidmann, T. et al., An Indicator Gene to Demonstrate Intracellular Transposition of Defective Retroviruses, Proc. Natl. Acad. Sci. USA, Apr. 1988, vol. 85, pp. 2219-2223.

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