DNA for the repressible and inducible expression of foreign gene

Details DNA for the repressible and inducible expression of foreign gene DNA for the repressible and inducible expression of foreign gene

1988-03-31

1990-10-16

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 712, 4351723, 435320, 536 27, 935 6, 935 8, 935 39, C12P 2100, C12N 1500, C07H 2104

Patent

active

049634828

ABSTRACT:
The present invention provides a recombinant DNA, wherein it contains the consensus sequence: ##STR1## and a promoter which has the DNA sequence GGN.sub.10 GC. The present invention also provides a process for the isolation of this recombinant DNA, wherein a DNA sequence containing the consensus sequence is identified from a gene bank of a micro-organism, which contains a gene which is repressible by oxygen and inducible by formate under anaerobic conditions, isolated and combined with an appropriate promotor according to known methods.
This recombinant gene can be used for the inducible and repressible expression of a foreign gene by bringing about the induction by formate under anaerobic conditions and the repression by oxygen.

REFERENCES:
Birkmann et al., Arch Microbiol 148:44-51, (1987).
Hirschman et al; Proc. Natl. Acad. Sci. USA, 82:7525, (1985).
Birkmann et al., Mol. Gen. Genet 210:535-542, (1987).
Reitzer et al., Cell 45:785-792.
Buck et al., Nucl. Acids Res, 13(21): 7621-7636, (1985).
Gussin et al., Ann. Rev. Genet 20:567-591, (1986).

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