Chemistry: molecular biology and microbiology – Vector – per se
Patent
1987-11-17
1992-09-08
Martinell, James
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 4351723, 4352523, 43525233, 536 27, 935 40, 935 41, 935 45, C12P 1571, C12P 1500, C12N 2100
Patent
active
051457822
ABSTRACT:
A DNA expression vector is described which is derived from the highly efficient trp operon. The expression vector provides for the direct expression of an inserted gene or cDNA. Using the expression vector described herein, it is possible to obtain the protein coded by the gene or cDNA directly and not as a fusion protein. The expression vector comprises the promoter, operator and leader ribosomal binding site of the trp operon.
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Burrell et al; Nature 279: 43 (1979).
Bertrand, K. et al, Science 189:22-26 (1975) "New features of the Regulation of the Tryptophan Operon".
Bennett, G. N. et al, Proc Natl Acad Sci (USA) 73:2351-2355 (1976) "Nucleotide sequence of region preceding trp mRNA initiation site and its role in promoter and operator function".
Sutcliffe, J. G. et al, Plasmid Cloning Vectors in Genetic Engineering (1978) by CRC Press, Inc., pp. 83, 92, 94-96.
Roberts, J. R., Methods in Enzymology 68: 27-41 (1979), "Directory of Restriction Endonucleases".
Enger-Valk, B. E., Gene 9:69-85 (1980), "Construction of New Cloning Vehicles with Genes of the Tryptophan Operon of Escherichia coli as Genetic Markers".
Stauffer, G. V. et al, Proc Natl Acad Sci (USA) 75:4833-4837, "Single base-pair alterations in the Escherichi coli trp operon leader region that relieve transciption termination at the trp attenuator".
Fritsch, A. etal, C. R. Acad. Sc. Pairs Series D 287, 1453 (1978), "Virologie--Clonage du genome du virus de l'hepatite B dans Escherichia coli".
Watson, Molecular Biology of the Gene (3rd Edition), pp. 394-395.
Edman Jeffrey C.
Goodman Howard M.
Hallewell Robert A.
Rutter William J.
Martinell James
The Regents of the University of California
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