DNA expression vector and use thereof

Details DNA expression vector and use thereof DNA expression vector and use thereof

1988-12-19

1991-09-03

Martinell, James

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 435 698, 435 711, 435 712, 435161, 435255, 435256, 435320, 536 27, 935 14, 935 48, 935 69, C12N 934, C12N 116, C12N 118, C12N 1500, C12P 2100, C12P 706, C07H 2104

Patent

active

050454633

ABSTRACT:
A gene having a DNA sequence complementary to that of the glucoamylase polypeptide mRNA from a fungal species, preferably Aspergillus awamori, is prepared. The mRNA is an approximately 2.2 kilobase poly A RNA obtained from fungal cells grown under conditions of glucoamylase induction. Reverse transcription of the mRNA provides a glucoamylase probe used to identify genomic digest fragments containing glucoamylase gene regions, which are sequenced to locate the introns and exons. The genomic fragments are spliced together to form a gene having a DNA sequence with altered or deleted introns which codes for fungal glucoamylase protein and is capable, when correctly combined with a cleaved DNA expression vector, of expressing a non-native protein having glucoamylase enzyme activity upon transformation of a host organism by the vector. The host is preferably bacteria or yeast. The transformed yeast host may be used to produce ethanol.

REFERENCES:
patent: 3418211 (1968-12-01), Van Lanen et al.
patent: 3660236 (1972-05-01), Dworschack et al.
patent: 4374198 (1983-02-01), Miller et al.
patent: 4411994 (1983-10-01), Gilbert et al.
patent: 4536477 (1985-08-01), Katkocin et al.
Yamashita et al., Agric. Biol. Chem., 47, (11), 2689-2692, (1983).
Rutter, "Production of `Valuable` Proteins in Alternate Biologial Hosts", in Recombinant DNA and Genetic Experimentation, pp. 123-128, (J. Morgan and W. J. Whelan, eds., 1979).
Hitzeman et al.; Nature, 293:717, (1981).
Okazaki et al., "Preferential Synthesis of Extracellular Glucoamylase by Aspergillus niger: Stability of mRNA Associated with Membrane-Bound Polysomes in Nongrowing Mycelia", J. Ferment. Technol., 56:279, (1978).
Houghton et al., "The Complete Amino Acid Sequence of Human Fibroblast Interferon as Deduced Using Synthetic Oligodeoxyribonucleotide Primers of Reverse Transcriptase", Nucleic Acids Res., 8:2885, (1980).
Helling et al., "The Molecular Cloning of Genes--General Procedures", in Genetic Engineering, Chakrabarty, (ed.), CRC Press, Inc., 1978, pp. 1-30.
Rosenberg et al., "Regulatory Sequences Involved in the Promotion and Termination of RNA Transcription", Ann. Rev. Genet., 13:319, (1979).
Maxam et al., "A New Method for Sequencing DNA", Proc. Natl. Acad. Sci. USA, 74:560, (1977).
Biological Science, Second Ed., 1972, Keeton, W. W. Norton & Co., Inc., New York, pp. 738-742.
Kinnaird, J. H. et al., Gene, 20, 387, (1982).
Shoemaker et al., Biotechnology, vol. 1, #8, (Oct. 1983), pp. 691-696.
Svensson, B. et al., Carlsberg Res. Commun., 47, p. 55, (1982).
Tubb et al., Curr. Dev. Yeast Res., 5th ed., 75-79, (1981), (Proc. Int. Yeast Symp.).
Erratt et al., Curr. Dev. Yeast Res., 5th ed., 177-183, (1981), (Proc. Int. Yeast Symp.).
Biotechnology News, vol. 2, No. 19, (Oct. 1, 1982), p. 2, concerning work of P. Rogers, School of Biotechnology, Sydney, Australia.
Poster Session at Cetus-UCLA Symposium on Gene Expression, (Mar. 26 to Apr. 1, 1983) in Park City, Utah.
Poster Session at ASM Conference on Gene Manipulations in the Exploitation and Study of Fungi, (May 1, 1983) in South Bend, Ind.
Lineback et al., "Two Forms of Glucoamylase of Aspergillus niger", Arch. Biochem. Biophys., 134:539, (1969).
M. J. Bull, Progress in Industrial Microbiology, vol. 15, Elsevier Scientific Publishing Co., Amsterdam, 1979, pp. 117-125.

Affiliated with

Also associated with

Rating

DNA expression vector and use thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA expression vector and use thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA expression vector and use thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1008857