Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1992-02-07
1992-11-24
Schwartz, Richard A.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
4353201, C12N 1531
Patent
active
051663295
ABSTRACT:
Method for the site specific genomic modification of yeasts of the genus Pichia and novel DNA sequences useful therefore are provided. Pichia is transformed with a serially arranged linear DNA fragment comprising first and second insertable DNA fragments, which flank a marker gene. The insertable DNA sequences are homologous to defined portions of the Pichia genome and integrate by recombination at such defined sites. The resulting transformed organism contains the marker gene and any additional DNA sequences which are positioned between the insertable DNA fragments. In addition, the resulting transformed organism has either a disrupted or deleted sequence at the site of the genomic modification. Enhanced production of heterologous gene products is observed when using as the host for expression strains in which the alcohol oxidase gene has been disrupted. Upon disruption of the primary alcohol oxidase gene of Pichia, the existence of a second alcohol oxidase gene in Pichia was also discovered.
REFERENCES:
Ellis et al., "Isolation of Alcohol Oxidase and Two Other Methanol Regulatable Genes from the Yeast Pichia pastoris," MCB, 5(5):1111-1121 (May 1985).
Couderc et al., "Oxidation of methanol by the yeast, Pichia pastoris purification and properties of the alcohol oxidase", Agric. Biol. Chem. 44(10):2279-2289 (1980).
Johnson Michelle L.
Phillips J. E.
Phillips Petroleum Company
Schwartz Richard A.
LandOfFree
DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-922807